Roche LightCycler 480 II Real-time PCR Thermal Cycler
Original price was: $17,899.00.$7,899.00Current price is: $7,899.00.
The Roche LightCycler 480 II Real-Time PCR System is a proven qPCR platform designed for labs that need reliable performance, flexible throughput, and consistent results across routine and advanced assays. It’s widely used for gene expression analysis, genotyping, SNP detection, assay development, and high-volume sample processing where speed and repeatability matter.
In stock
Key benefits of the Roche LightCycler 480 optical system:
- Highest flexibility in the choice of fluorescence dyes and detection formats for all current PCR applications, including high resolution melting (HRM).
- Accurate data capture across the entire plate.
- New long-pass filter (705 nm detection).
- Enhanced multiplexing capabilities for 4-color hydrolysis probe and 3-color HybProbe probe assays with pre-defined templates.
- Optimal performance of dual color Hydrolysis Probes assay supported by universal color compensation objects for FAM / VIC or Universal ProbeLibrary dual color assays.
Products are for Life Science Research Use Only, not for use in diagnostic procedures, unless otherwise indicated.
Features:
- Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis. Use basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves.
- Benefit from cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity.Use a wide range of probe formats (e.g., FRET or HybProbe probes, SimpleProbe probes, hydrolysis probes) and fluorescent dyes (e.g., SYBR Green I, ResoLight Dye, FAM, HEX, Cy5, LightCycler® Red 610, LightCycler® Red 640, and LightCycler® CYAN 500).
- Choose the optimal type of multiwell plate (white or clear) to best fit your assay format, application, and workflow. Use the available adapter plate to also work with LightCycler® tube strips.
- Build a modular real-time PCR platform to meet your particular needs. Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, and benefit from additional user-friendly software modules.
- Extract meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines. Analyze true raw data without the need for passive reference dyes or normalization plates.
- Protect your data using a system compatible with 21 CFR part 11 requirements.Integrate the LightCycler® 480 System as part of a LIMS in combination with robotic pipetting and plate loading.
Application
Specifications:
- Dimensions (W x D x H): 24 in x 24 in x 21.5 in (60 cm x 60 cm x 54.5 cm)
- Weight: 121 lbs (55kg)
- Sample Number: 96/384
- Excitation Source: Broad-spectrum, high-intensity LED (390 – 710 nm)
- Excitation Filters: 440, 465, 498, 533, 618 nm (System II)
- Detection: CCD camera
- Detection Filters: 488, 510, 580, 610,640, 660 nm (System II)
- Measuring Mode: Real-time, online (continuous display of readings)
- Temperature Control: Peltier-based heating/cooling
- Run Time: <40 minutes (384-well PCR plate)
Roche LightCycler 480 II / 384 LED Real-time PCR Thermal Cycler System with 1.5.1.62 SP3 Software. Recently removed from a large lab closure. System is in good condition, powers up, and interfaces with the included PC running Windows 10 & 1.5.1.62 SP3 Instrument Software. PDF manuals are included with the software. Instrument passes all internal self-tests as shown. No further testing has been done. Set comes as pictured. Also included is the Driftcon II validation kit. No further accessories or components beyond what is shown are included. Please make sure this works for your needs before purchase.
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