{"id":640,"date":"2021-08-27T05:41:03","date_gmt":"2021-08-27T05:41:03","guid":{"rendered":"https:\/\/dipylonmedical.com\/?post_type=product&#038;p=640"},"modified":"2024-11-16T01:57:02","modified_gmt":"2024-11-16T01:57:02","slug":"lightcycler-96","status":"publish","type":"product","link":"https:\/\/dipylonmedical.com\/nl\/product\/lightcycler-96\/","title":{"rendered":"Roche LightCycler 96 Real Time PCR Systeem"},"content":{"rendered":"<p style=\"text-align: justify;\">LightCycler 96 Instrument is a real-time PCR system for rapidly cycling up to 96 samples. It is an optical detection system that allows flexible detection of sequence-dependent probes, such as hydrolysis and sequence-independent dyes (<em>i.e.,\u00a0<\/em>SYBR Green I). Multiplex and multicolor capabilities permit using up to four different fluorescent dyes.<\/p>\n<p style=\"text-align: justify;\"><b>LightCycler 96<\/b>\u00a0real-time PCR system\u00a0<b>from Roche (Germany)<\/b>\u00a0allows you to perform all major types of real-time PCR analysis, including the qualitative and quantitative determination of target nucleic acid sequences, genotyping with TaqMan probes, gene expression analysis, and HRM analysis already in the basic configuration of the device.<\/p>\n<p style=\"text-align: justify;\">\n<style>.embed-container { position: relative; padding-bottom: 56.25%; height: 0; overflow: hidden; max-width: 100%; } .embed-container iframe, .embed-container object, .embed-container embed { position: absolute; top: 0; left: 0; width: 100%; height: 100%; }<\/style>\n<\/p>\n<div class=\"embed-container\" style=\"text-align: justify;\"><iframe src=\"https:\/\/www.youtube.com\/embed\/8eOPYJ90r5M\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/div>\n<h2 class=\"p1\" style=\"text-align: justify;\"><span class=\"s1\"><b>LightCycler 96 Real-Time PCR System \u2013 A Brief<\/b><\/span><\/h2>\n<p class=\"p2\" style=\"text-align: justify;\"><span class=\"s1\">PCR systems find prevalent usage in research labs nowadays. Be it their performance, size, or anything related to the machine, and it intrigues the researchers to study more through them. If you are looking for a medium-throughout and lightweight system, the <b>LightCycler 96 Real-Time PCR Systeem<\/b>\u00a0is a perfect choice. Read to know more about this PCR system from Roche and its benefits.\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\">The LightCycler 96 module for high-resolution melting analysis will suit human genetic disease research applications. It enables easy and fast detection of known and unknown gene mutations, including single-nucleotide polymorphisms, deletions, and methylated regions.<\/p>\n<p class=\"p2\" style=\"text-align: justify;\"><span class=\"s1\"><b>LightCycler 96 Specifications:\u00a0<\/b><\/span><\/p>\n<ul class=\"ul1\" style=\"text-align: justify;\">\n<li class=\"li2\"><span class=\"s1\">Measurement \u2013 40 x 40 x 53 cm\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Temperature Range \u2013 37-98 degrees\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Detection \u2013 Cooled CCD Camera\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Sensitivity \u2013 one copy\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Wells \u2013 96\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Purpose \u2013 COVID-19 Diagnosis\u00a0<\/span><\/li>\n<\/ul>\n<h3 class=\"p2\" style=\"text-align: justify;\"><span class=\"s1\"><b>LightCycler 96 Features:\u00a0<\/b><\/span><\/h3>\n<p class=\"p2\" style=\"text-align: justify;\"><span class=\"s1\">De\u00a0<b>LightCycler 96 Real-Time PCR system<\/b>\u00a0finds its application in detecting the coronavirus using the SARS-CoV-2 PCR detection. The machine achieves the intended results with the help of the following features:\u00a0<\/span><\/p>\n<ol class=\"ol1\" style=\"text-align: justify;\">\n<li class=\"li2\"><span class=\"s1\">It comes with a plate-based system with 96 unique wells.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Its high-performance temperature gradient is efficient for assay optimization.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">It provides a 4-plex multicolor operation.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">This machine has one copy sensitivity and a dynamic range of 10 logs.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">It is a standalone system and is integrable with a personal computer.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">A separate instrument software with a touchscreen mechanism. It is free of cost and can loan on any PC.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">It has a runtime of less than an hour.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">It can perform relative and absolute quantification, endpoint genotyping, and melting curve analysis.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">This PCR machine uses intuitive and state-of-the-art software for phenomenal results and a convenient interface.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Its PC analysis software is also free of charge and can be easily loaded on any personal computer.\u00a0<\/span><\/li>\n<\/ol>\n<h2 class=\"p2\" style=\"text-align: justify;\"><span class=\"s1\"><b>LightCycler 96 Advantages:\u00a0<\/b><\/span><\/h2>\n<p class=\"p2\" style=\"text-align: justify;\"><span class=\"s1\">As mentioned above, the main purpose of the\u00a0<b>LightCycler 96 Real-Time PCR system<\/b>\u00a0is COVID-19 detection. It eases the detection process and provides efficient results each time. The following are some of the many advantages of this PCR system.\u00a0<\/span><\/p>\n<ol class=\"ol1\" style=\"text-align: justify;\">\n<li class=\"li2\"><span class=\"s1\">This machine combines sturdy glass fibers with higher intensity and broad-spectrum LEDs to maximize the device&#8217;s sensitivity. It can detect all the wells simultaneously in less than a second.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">It provides free-to-use software that fits according to the operator\u2019s needs like the following:\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">As a standalone machine. We can use a flash drive to transfer the data.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">We can directly connect the machine to a PC.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">We can connect the machine to any PC using a LAN network or Ethernet connectivity.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Its state-of-the-art analysis methods help in extracting only useful and meaningful data. These in turn export the obtained results in an RDML format (ready to publish).\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Its cutting-edge low-mass thermal block design accounts for the machine\u2019s homogeneity. Also, they provide broad temperature ranges and accessible gradient features.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">This machine uses an electroforming production process to fit the 96-well plate exactly to the block, which is a perfect balance between the positive (block) and negative (plate). This method also avoids air cushions that can potentially disturb the readings.\u00a0<\/span><\/li>\n<li class=\"li2\"><span class=\"s1\">Its specific Application and Instrument Software provides an easy analysis workflow. It provides convenient import and export functions, email notifications after every run, a server-based network interface, and flexible online monitoring.\u00a0<\/span><\/li>\n<\/ol>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-642 size-large\" src=\"https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-1131x800.png\" alt=\"LightCycler 96 specifications\" width=\"1020\" height=\"721\" srcset=\"https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-1131x800.png 1131w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-300x212.png 300w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-1000x708.png 1000w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-565x400.png 565w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-768x543.png 768w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-1536x1087.png 1536w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications-600x425.png 600w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-specifications.png 1754w\" sizes=\"auto, (max-width: 1020px) 100vw, 1020px\" \/><\/p>\n<h3 style=\"text-align: justify;\"><b>Main characteristics<\/b><\/h3>\n<table border=\"1\" width=\"100%\" cellspacing=\"0\" cellpadding=\"5\">\n<tbody>\n<tr>\n<td>Control<\/td>\n<td>Built-in intuitive touchscreen \/ PC control<\/td>\n<\/tr>\n<tr>\n<td>Excitation channels, nm<\/td>\n<td>470, 533, 577, 645<\/td>\n<\/tr>\n<tr>\n<td>Detection channels, nm<\/td>\n<td>514, 572, 620, 697<\/td>\n<\/tr>\n<tr>\n<td>Recommended dyes<\/td>\n<td>Fluorescent labeled probes: FAM, HEX, VIC, Joe, Yellow555, ROX, Red610, TexasRed, Cy5<\/td>\n<\/tr>\n<tr>\n<td>Device dimensions (WxDxH, weight)<\/td>\n<td>40cm \u00d7 40cm \u00d7 53cm;\u00a025 Kg<\/td>\n<\/tr>\n<tr>\n<td>Expendable materials<\/td>\n<td>Standard low-profile:<br \/>\n&#8211; 96-well plates with or without a \u201c<br \/>\nhalf-\u00a0skirt\u201d\u00a0&#8211; stripped tubes of 8 pcs.<\/td>\n<\/tr>\n<tr>\n<td>The volume of the reaction mixture<\/td>\n<td>10-50 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>Nauwkeurigheid van temperatuuronderhoud<\/td>\n<td>\u00b1 0.2 \u00b0 C<\/td>\n<\/tr>\n<tr>\n<td>Temperature homogeneity<\/td>\n<td>\u00b1 0.3 \u00b0 C<\/td>\n<\/tr>\n<tr>\n<td>Temperature range<\/td>\n<td>37\u201398 \u00b0 C<\/td>\n<\/tr>\n<tr>\n<td>Heating rate<\/td>\n<td>4.4 \u00b0 C \/ sec<\/td>\n<\/tr>\n<tr>\n<td>Cooling rate<\/td>\n<td>2.2 \u00b0 C \/ sec<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2 style=\"text-align: justify;\">Inclusief:<\/h2>\n<ul style=\"text-align: justify;\">\n<li>High Pure Viral RNA Kit &#8211; 100 purifications<\/li>\n<li>Lightmix Modular SARS-CoV (COVID-19) TIB MOLBIOL<\/li>\n<li>White stripped tubes for real-time PCR, with caps \/ LightCycler 8-Tube Strips (white), 120 strips \/ pack<\/li>\n<li>96-well clear plate (LightCycler480 Multiwell Plate 96, clear)<\/li>\n<li>96-well plate (LightCycler 480 Multiwell Plate 96)<\/li>\n<li>Greiner Bio-One Roche LightCycler Compatible 96-Well Microplates<\/li>\n<li>LightCycler RNA Amplification Kit HybProbe &#8211; 1 kit (for 96 reactions of 20 uL final reaction volume)<\/li>\n<li>LightCycler RNA Master SYBR Green I &#8211; 1 kit (for 96 reactions of 20 uL final reaction volume)<\/li>\n<li>High Pure RNA Isolation Kit<\/li>\n<li>2 lightcycler 96 quick guides:<br \/>\n-system installation -programming and running an experiment<br \/>\n1 lightcycler 96 usb drive containing:<br \/>\n-executable for the software -release notes -license texts -lightcycler 96 system guides decontamination and shipment preparation -demo data and experiment templates provided by roche<\/li>\n<li>1 mains power cable (eu)<\/li>\n<li>1 mains power cable (us)<\/li>\n<li>1 lan cable (3 m)<\/li>\n<li>2 ventilation dust filters<\/li>\n<li>1 fuse package fuse 5&#215;20 t8ah 250v ulr\/iec<\/li>\n<li>1 sealing foil applicator<\/li>\n<\/ul>\n<h2 style=\"text-align: justify;\">Referentielink<\/h2>\n<ul style=\"text-align: justify;\">\n<li><a href=\"https:\/\/diagnostics.roche.com\/global\/en\/products\/instruments\/lightcycler-96.html\" target=\"_blank\" rel=\"noopener\">Fabrikant informatie<\/a><\/li>\n<li><a href=\"https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/08\/LightCycler-96-Real-Time-PCR-Brochure-DIPYLON-MEDICAL.pdf\">Brochure<\/a><\/li>\n<\/ul>\n<p style=\"text-align: justify;\">","protected":false},"excerpt":{"rendered":"<p><span class=\"s1\"><b>LightCycler 96 Real-Time PCR system<\/b>\u00a0finds its application in detecting the coronavirus using the SARS-CoV-2 PCR detection.<\/span><\/p>","protected":false},"featured_media":643,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[70],"product_tag":[100],"class_list":["post-640","product","type-product","status-publish","has-post-thumbnail","product_cat-pcr-thermal-cycler","product_tag-lightcycler-96","first","instock","sale","shipping-taxable","purchasable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product\/640","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/comments?post=640"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/media\/643"}],"wp:attachment":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/media?parent=640"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product_brand?post=640"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product_cat?post=640"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product_tag?post=640"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}