{"id":1286,"date":"2026-04-02T02:30:11","date_gmt":"2026-04-02T02:30:11","guid":{"rendered":"https:\/\/dipylonmedical.com\/?post_type=product&#038;p=1286"},"modified":"2026-04-02T02:31:40","modified_gmt":"2026-04-02T02:31:40","slug":"roche-lightcycler-480-ii-384-led-real-time-pcr","status":"publish","type":"product","link":"https:\/\/dipylonmedical.com\/nl\/product\/roche-lightcycler-480-ii-384-led-real-time-pcr\/","title":{"rendered":"Roche LightCycler 480 II Real-time PCR Thermocycler"},"content":{"rendered":"<div class=\"rte-styles margin--b24\">\n<div class=\"js-overviewtext-wrapper\"><strong>Roche LightCycler 480<\/strong> Instrument II is a rapid, plate-based, thermal block cycler with integrated real-time, online detection capabilities. Based on a comprehensive improvement of Peltier-based technology incorporated in a silver block, the instrument provides extraordinary well-to-well temperature homogeneity and inter-well, inter-cycle reproducibility. The special arrangement of optical components in the LightCycler<sup>\u00ae<\/sup> 480 Instrument II ensures uniform signal collection across the plate and makes analysis independent of sample position.<\/div>\n<div><\/div>\n<div><\/div>\n<div class=\"js-overviewtext-wrapper\">The LightCycler<sup>\u00ae<\/sup> 480 System setup enables the use of all current probe formats (e.g., SYBR Green I, ResoLight dye for high-resolution melting, hydrolysis probes, HybProbe probes, SimpleProbe probes), providing a solution for fast and precise qualitative or quantitative detection of nucleic acids, genotyping, and mutation scanning. Channels and filters can be combined in different ways to provide flexibility for mono-color and multiplex assays.<\/div>\n<div><\/div>\n<div><\/div>\n<div class=\"js-overviewtext-wrapper\">The pre-installed Roche LightCycler 480\u00a0Software provides an intuitive, easy-to-navigate user interface for real-time PCR programming, data capture, and data analysis. It facilitates highly versatile, extensive data analysis for studying gene expression and genetic variation. The LightCycler<sup>\u00ae<\/sup> 480 Software enables users to quickly customize the system to meet various application requirements, from basic manual analysis to automated data evaluation using the software macro feature.<\/div>\n<div><\/div>\n<\/div>\n<div>\n<div style=\"height: 0px; overflow: hidden; padding-top: 56.25%; position: relative; width: 100%;\"><iframe style=\"position: absolute; top: 0px; left: 0px; width: 100%; height: 100%;\" title=\"YouTube video player\" src=\"https:\/\/tube.rvere.com\/embed?v=Yxce4K0xsec\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><br \/>\n<\/iframe><\/div>\n<\/div>\n<div class=\"rte-styles margin--b24\">\n<div>\n<h2>Key benefits of the Roche LightCycler 480\u00a0optical system:<\/h2>\n<ul>\n<li>Highest flexibility in the choice of fluorescence dyes and detection formats for all current PCR applications, including high resolution melting (HRM).<\/li>\n<li>Accurate data capture across the entire plate.<\/li>\n<li>New long-pass filter (705 nm detection).<\/li>\n<li>Enhanced multiplexing capabilities for 4-color hydrolysis probe and 3-color HybProbe probe assays with pre-defined templates.<\/li>\n<li>Optimal performance of dual color Hydrolysis Probes assay supported by universal color compensation objects for FAM \/ VIC or Universal ProbeLibrary dual color assays.<\/li>\n<\/ul>\n<p>Products are for Life Science Research Use Only, not for use in diagnostic procedures, unless otherwise indicated.<\/p>\n<h3><strong>Features:<\/strong><\/h3>\n<ul>\n<li>Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis. Use basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves.<\/li>\n<li>Benefit from cutting-edge thermal block and optical technology that leads to excellent well-to-well homogeneity and maximized detection sensitivity.Use a wide range of probe formats (e.g., FRET or HybProbe probes, SimpleProbe probes, hydrolysis probes) and fluorescent dyes (e.g., SYBR Green I, ResoLight Dye, FAM, HEX, Cy5, LightCycler\u00ae Red 610, LightCycler\u00ae Red 640, and LightCycler\u00ae CYAN 500).<\/li>\n<li>Choose the optimal type of multiwell plate (white or clear) to best fit your assay format, application, and workflow. Use the available adapter plate to also work with LightCycler\u00ae tube strips.<\/li>\n<li>Build a modular real-time PCR platform to meet your particular needs. Flexibly switch between plate formats and thermal block cyclers in just a few minutes with no recalibration time, and benefit from additional user-friendly software modules.<\/li>\n<li>Extract meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines. Analyze true raw data without the need for passive reference dyes or normalization plates.<\/li>\n<li>Protect your data using a system compatible with 21 CFR part 11 requirements.Integrate the LightCycler\u00ae 480 System as part of a LIMS in combination with robotic pipetting and plate loading.<\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div class=\"header-tag\">\n<h3 class=\"pim-h3\">Application<\/h3>\n<\/div>\n<div class=\"rte-styles margin--b24\">\n<div class=\"js-overviewtext-wrapper\">The LightCycler<sup>\u00ae<\/sup>\u00a0480 System is a high-performance, high-throughput PCR platform (96- or 384-multiwell plates) that provides various methods for gene detection, gene expression, and genetic variation analysis. The system features the LightCycler<sup>\u00ae<\/sup> 480 Instrument, a versatile, plate-based real-time PCR device that supports mono- or multicolor applications, as well as multiplex protocols. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as a robotically controlled, automated high-throughput solution.<\/div>\n<\/div>\n<h3><strong>Specifications:<\/strong><\/h3>\n<ul>\n<li>Dimensions (W x D x H): 24 in x 24 in x 21.5 in (60 cm x 60 cm x 54.5 cm)<\/li>\n<li>Weight: 121 lbs (55kg)<\/li>\n<li>Sample Number: 96\/384<\/li>\n<li>Excitation Source: Broad-spectrum, high-intensity LED (390 \u2013 710 nm)<\/li>\n<li>Excitation Filters: 440, 465, 498, 533, 618 nm (System II)<\/li>\n<li>Detection: CCD camera<\/li>\n<li>Detection Filters: 488, 510, 580, 610,640, 660 nm (System II)<\/li>\n<li>Measuring Mode: Real-time, online (continuous display of readings)<\/li>\n<li>Temperature Control: Peltier-based heating\/cooling<\/li>\n<li>Run Time: &lt;40 minutes (384-well PCR plate)<\/li>\n<\/ul>\n<p>Roche LightCycler 480 II \/ 384 LED Real-time PCR Thermal Cycler System with 1.5.1.62 SP3 Software. Recently removed from a large lab closure. System is in good condition, powers up, and interfaces with the included PC running Windows 10 &amp; 1.5.1.62 SP3 Instrument Software. PDF manuals are included with the software. Instrument passes all internal self-tests as shown. No further testing has been done. Set comes as pictured. Also included is the Driftcon II validation kit. No further accessories or components beyond what is shown are included. Please make sure this works for your needs before purchase.<\/p>","protected":false},"excerpt":{"rendered":"<p>The <strong data-start=\"335\" data-end=\"384\">Roche LightCycler 480 II Real-Time PCR System<\/strong> is a proven qPCR platform designed for labs that need reliable performance, flexible throughput, and consistent results across routine and advanced assays. It\u2019s widely used for <strong data-start=\"562\" data-end=\"590\">gene expression analysis<\/strong>, <strong data-start=\"592\" data-end=\"606\">genotyping<\/strong>, <strong data-start=\"608\" data-end=\"625\">SNP detection<\/strong>, <strong data-start=\"627\" data-end=\"648\">assay development<\/strong>, and high-volume sample processing where speed and repeatability matter.<\/p>","protected":false},"featured_media":1287,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[70],"product_tag":[204],"class_list":["post-1286","product","type-product","status-publish","has-post-thumbnail","product_cat-pcr-thermal-cycler","product_tag-roche-lightcycler-480","first","instock","sale","shipping-taxable","purchasable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product\/1286","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/comments?post=1286"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/media\/1287"}],"wp:attachment":[{"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/media?parent=1286"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product_brand?post=1286"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product_cat?post=1286"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/dipylonmedical.com\/nl\/wp-json\/wp\/v2\/product_tag?post=1286"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}