{"id":980,"date":"2021-11-04T14:26:04","date_gmt":"2021-11-04T14:26:04","guid":{"rendered":"https:\/\/dipylonmedical.com\/?post_type=product&#038;p=980"},"modified":"2025-01-20T02:19:32","modified_gmt":"2025-01-20T02:19:32","slug":"illumina-miniseq","status":"publish","type":"product","link":"https:\/\/dipylonmedical.com\/ja\/product\/illumina-miniseq\/","title":{"rendered":"\u30a4\u30eb\u30df\u30ca MiniSeq DNA\/RNA \u30b7\u30b9\u30c6\u30e0"},"content":{"rendered":"<p style=\"text-align: justify;\">Illumina MiniSeq system offers the industry standard and high-quality requirements corresponding sequencing technology from Illumina and the ease of use of a user-friendly, inexpensive desktop system.<\/p>\n<p style=\"text-align: justify;\">The MiniSeq system offers the power and confidence of Illumina technology in an affordable sequencing system. It features cost-effective sequencing, even for a low number of samples. Its streamlined workflow enables rapid DNA and RNA sequencing. The small size allows it to be perfectly adapted to laboratories, without the need for specialized auxiliary equipment.<\/p>\n<p style=\"text-align: justify;\">The MiniSeq system offers flexibility between applications, allowing researchers to easily transition between sequencing projects for DNA and RNA applications, including signal transduction pathway profiling, hematology and solid tumor molecular profiling, and mutation analysis in the germ line. Optimized data analytics workflows are available for a growing application ecosystem.<\/p>\n<p style=\"text-align: justify;\">The Illumina MiniSeq system combines loading and unloading operation with an intuitive user interface for fast and easy NGS workflow. The MiniSeq system integrates clonal amplification, sequencing by synthesis, and so-called base in a single instrument, eliminating the need for additional equipment. Simply load the flow cell and reagent cartridge into the MiniSeq system and sequence with the touch of a button.<\/p>\n<p style=\"text-align: justify;\">\n<style>.embed-container { position: relative; padding-bottom: 56.25%; height: 0; overflow: hidden; max-width: 100%; } .embed-container iframe, .embed-container object, .embed-container embed { position: absolute; top: 0; left: 0; width: 100%; height: 100%; }<\/style>\n<\/p>\n<div class=\"embed-container\" style=\"text-align: justify;\"><iframe src=\"https:\/\/www.youtube.com\/embed\/JBmOWNmj4Gk\" frameborder=\"0\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/div>\n<h2 style=\"text-align: justify;\">Illumina MiniSeq Features:<\/h2>\n<ul style=\"text-align: justify;\">\n<li>system performance scalability (2-7.5Gb)<\/li>\n<li>full sequencing cycle from 4 to 24h<\/li>\n<li>fully automatic generation of clusters on board the system<\/li>\n<li>fully automatic data analysis on board the system<\/li>\n<li>high quality of the obtained data<\/li>\n<li>no inconsistency of results with homopolymers<\/li>\n<li>fully automatic paired-end sequencing<\/li>\n<\/ul>\n<h3 style=\"text-align: justify;\">Simple and affordable next-generation sequencer<\/h3>\n<p style=\"text-align: justify;\">Illumina quality and reliability in a compact, affordable sequencer!\u00a0MiniSeq allows your laboratory to make NGS a routine method.\u00a0The MiniSeq is suitable for laboratories of all sizes &#8211; for both large and small sample flows.<\/p>\n<p style=\"text-align: justify;\">The MiniSeq combines the best features of the MiSeq and NextSeq sequencers: the use of disposable reagent cartridges and a simplified fluidics system.\u00a0MiniSeq provides exceptional speed and, with its compact size, allows you to get up to 7.5 GB of data and about 25 million reads in just a day.<\/p>\n<h3 style=\"text-align: justify;\">Affordable tabletop sequencer<\/h3>\n<p style=\"text-align: justify;\">The MiniSeq is one of the most affordable sequencers offering the performance and reliability of proven Illumina NGS technology.\u00a0With the MiniSeq, sequencing even small numbers of samples is cost effective.\u00a0Its streamlined workflow enables fast sequencing of both DNA and RNA.\u00a0The small size makes the MiniSeq easy to fit in the laboratory without the need for specialized accessories.<\/p>\n<h3 style=\"text-align: justify;\">System Applications<\/h3>\n<p style=\"text-align: justify;\">The MiniSeq system allows researchers to easily switch between DNA and RNA sequencing projects, including signaling pathway profiling, solid and hematologic tumor profiling, and germline mutation analysis.<\/p>\n<h3 style=\"text-align: justify;\">Workflow: A Simple Path from Libraries to Results<\/h3>\n<p style=\"text-align: justify;\">The MiniSeq system combines ease of use with an intuitive user interface.\u00a0Cluster generation and sequencing are combined in one compact benchtop sequencer, so you don&#8217;t need any additional hardware.\u00a0Simply load the flowcell and reagent cartridge into the MiniSeq and start a sequence with the push of a button.<\/p>\n<h3 style=\"text-align: justify;\">Convenient and intuitive software<\/h3>\n<p style=\"text-align: justify;\">Monitoring and analysis of the sequencer run is performed using built-in software that does not require an Internet connection.\u00a0The device itself will make de novo assembly or alignment to the reference genome and variant calling for you.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-982 size-large\" src=\"https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-1400x510.jpeg\" alt=\"Illumina-MiniSeq-Lab\" width=\"1020\" height=\"372\" srcset=\"https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-1400x510.jpeg 1400w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-scaled-300x109.jpeg 300w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-scaled-1000x364.jpeg 1000w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-800x292.jpeg 800w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-768x280.jpeg 768w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-1536x560.jpeg 1536w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-2048x746.jpeg 2048w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-50x18.jpeg 50w, https:\/\/dipylonmedical.com\/wp-content\/uploads\/2021\/11\/Illumina-MiniSeq-Lab-600x219.jpeg 600w\" sizes=\"auto, (max-width: 1020px) 100vw, 1020px\" \/><\/p>\n<h3 style=\"text-align: justify;\">These characteristics make the Illumina MiniSeq suitable for the following tasks:<\/h3>\n<p style=\"text-align: justify;\"><b>1) Oncology<\/b><\/p>\n<p style=\"text-align: justify;\">&#8211; analysis of somatic mutations in solid tumors (TruSight Tumor 15 panels, AmpliSeq panel line) and myeloid neoplasms (TruSight Myeloid)<br \/>\n&#8211; analysis of predisposition to oncological diseases (TruSight Cancer, AmpliSeq panels)<br \/>\n&#8211; cancer transcriptome-Pan-Cancer research and fusion (TruSight RNA Fusion, Archer Dx panel line)<\/p>\n<p style=\"text-align: justify;\"><b>2) RNA sequencing<\/b><\/p>\n<p style=\"text-align: justify;\">&#8211; custom TruSeq Targeted RNA Expression panels to assess gene expression<br \/>\n&#8211; RNA-Seq microorganisms, sequencing of RNA viruses<br \/>\n&#8211; studying small RNA (TruSeq Small RNA)<\/p>\n<p style=\"text-align: justify;\"><b>3) Targeted sequencing<\/b><\/p>\n<p style=\"text-align: justify;\">&#8211; a wide range of panels for any task.<\/p>\n<p style=\"text-align: justify;\"><b>4) Whole-genome sequencing of small genomes (up to 5 Mb)<\/b><\/p>\n<h3 class=\"caption\"><strong>\u4ed5\u69d8<\/strong><\/h3>\n<div class=\"container-fluid\">\n<div class=\"technical\">\n<div>\n<p>Approximate response time and performance (cluster generation and sequencing)<\/p>\n<\/div>\n<table border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<thead>\n<tr>\n<th><strong>Reading length (bp)<\/strong><\/th>\n<th><strong>total time\u00a0<sup>*<\/sup><\/strong><\/th>\n<th><strong>performance<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td colspan=\"3\">MINISEQ SYSTEM HIGH-OUTPUT KIT<\/td>\n<\/tr>\n<tr>\n<td>1 \u00d7 75<\/td>\n<td>approx. 7 hours<\/td>\n<td>1.65-1.875 Gb<\/td>\n<\/tr>\n<tr>\n<td>2 \u00d7 75<\/td>\n<td>approx. 13 hours<\/td>\n<td>3.3-3.75 Gb<\/td>\n<\/tr>\n<tr>\n<td>2 \u00d7 150<\/td>\n<td>approx. 24 hours<\/td>\n<td>6.6-7.5 Gb<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\">MINISEQ SYSTEM MID-OUTPUT KIT<\/td>\n<\/tr>\n<tr>\n<td>2 x 150<\/td>\n<td>approx. 17 hours<\/td>\n<td>2.1-2.4 Gb<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<table border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td><strong>Number of readings filtered<\/strong><\/td>\n<td><strong>The quality of the readings<\/strong><\/td>\n<\/tr>\n<tr>\n<td>\n<table border=\"0\" cellspacing=\"0\" cellpadding=\"0\" align=\"left\">\n<thead>\n<tr>\n<th><strong>type of reaction<\/strong><\/th>\n<th><strong>number of readings filtered<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>Single readings<\/td>\n<td>22-25 million<\/td>\n<\/tr>\n<tr>\n<td>Paired end readings<\/td>\n<td>44-50 million<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/td>\n<td>\n<table border=\"0\" cellspacing=\"0\" cellpadding=\"0\" align=\"left\">\n<thead>\n<tr>\n<th><strong>reading length<\/strong><\/th>\n<th><strong>base pairs above q30<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>1 \u00d7 75 bp<\/td>\n<td>&gt; 85%<\/td>\n<\/tr>\n<tr>\n<td>2 \u00d7 75 bp<\/td>\n<td>&gt; 85%<\/td>\n<\/tr>\n<tr>\n<td>2 \u00d7 150 bp<\/td>\n<td>&gt; 80%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3 class=\"caption\">\u542b\u3081\u308b<\/h3>\n<ul>\n<li>Agilent 2100 Bioanalyzer<\/li>\n<li>Rapid Reagent Kit (100 cycles)<\/li>\n<li>High Output Reagent Kit (75-cycles)<\/li>\n<li>High Output Reagent Kit (150-cycles)<\/li>\n<\/ul>\n<h3 class=\"caption\">Documents<\/h3>\n<ul class=\"docs\">\n<li class=\"lnk\"><a href=\"https:\/\/emea.illumina.com\/systems\/sequencing-platforms\/comparison-tool.html?langsel=\/pl\/\" target=\"_blank\" rel=\"noopener\">Comparison of sequencing systems<\/a><\/li>\n<li class=\"lnk\"><a href=\"https:\/\/emea.illumina.com\/systems\/sequencing-platforms.html\" target=\"_blank\" rel=\"noopener\">Comparison of sequencing systems<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/documents\/products\/brochures\/brochure_sequencing_systems_portfolio.pdf\" target=\"_blank\" rel=\"noopener\">Sequencing Portfolio brochure<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/brochures\/miniseq-brochure-web.pdf\" target=\"_blank\" rel=\"noopener\">MiniSeq system brochure<\/a><\/li>\n<li class=\"lnk\"><a href=\"https:\/\/science-docs.illumina.com\/documents\/Instruments\/miniseq-system-spec-sheet-770-2015-039\/miniseq-system-spec-sheet-770-2015-039.pdf\" target=\"_blank\" rel=\"noopener\">Specification of the MiniSeq system<\/a><\/li>\n<li class=\"lnk\"><a href=\"https:\/\/emea.illumina.com\/systems\/sequencing-platforms\/miniseq\/specifications.html\" target=\"_blank\" rel=\"noopener\">Specification of the MiniSeq system<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/brochures\/oncology-flipbook-770-2015-050-web.pdf\" target=\"_blank\" rel=\"noopener\">MiniSeq Oncology brochure<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/brochures\/molecular-biology-flipbook-770-2015-051-web.pdf\" target=\"_blank\" rel=\"noopener\">MiniSeq brochure molecular biology<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/appnotes\/profiling-cancer-associated-genetic-variants-on-the-miniseq-system-application-note-770-2015-043.pdf\" target=\"_blank\" rel=\"noopener\">Application note &#8211; tumor profiling<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/appnotes\/complete-small-rna-sequencing-solutions-on-the-miniseq-system-application-note-770-2015-045.pdf\" target=\"_blank\" rel=\"noopener\">Application note &#8211; miRNA profiling<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/appnotes\/targeted-resequencing-solutions-on-the-miniseq-system-application-note-770-2015-047.pdf\" target=\"_blank\" rel=\"noopener\">Application note &#8211; targeted resequencing<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/appnotes\/targeted-rna-expression-solutions-on-the-miniseq-system-application-note-770-2015-046.pdf\" target=\"_blank\" rel=\"noopener\">Application burrow &#8211; expression profiling<\/a><\/li>\n<li class=\"lnk\"><a href=\"http:\/\/www.illumina.com\/content\/dam\/illumina-marketing\/documents\/products\/technotes\/technote_coverage_calculation.pdf\" target=\"_blank\" rel=\"noopener\">Technical Note &#8211; Establishing Sequencing Coverage<\/a><\/li>\n<li class=\"lnk\"><a href=\"https:\/\/emea.illumina.com\/systems\/sequencing-platforms\/miniseq\/applications.html\" target=\"_blank\" rel=\"noopener\">Main applications and methods<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p style=\"text-align: justify;\">Illumina MiniSeq system offers the industry standard and high-quality requirements corresponding sequencing technology from Illumina and the ease of use of a user-friendly, inexpensive desktop system.<\/p>","protected":false},"featured_media":983,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[80],"product_tag":[180],"class_list":["post-980","product","type-product","status-publish","has-post-thumbnail","product_cat-dna-rna-purification","product_tag-illumina-miniseq","first","instock","sale","shipping-taxable","purchasable","product-type-simple"],"_links":{"self":[{"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/product\/980","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/comments?post=980"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/media\/983"}],"wp:attachment":[{"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/media?parent=980"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/product_brand?post=980"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/product_cat?post=980"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/dipylonmedical.com\/ja\/wp-json\/wp\/v2\/product_tag?post=980"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}